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Image Search Results
Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Article Title: Association of Immune Response to Endothelial Cell Growth Factor With Early Disseminated and Late Manifestations of Lyme Disease but Not Posttreatment Lyme Disease Syndrome
doi: 10.1093/cid/civ638
Figure Lengend Snippet: Antibody reactivity to endothelial cell growth factor (ECGF). A, Level and frequency of immunoglobulin G (IgG) reactivity to ECGF, as determined with enzyme-linked immunosorbent (ELISA), in healthy controls without a history or serologic evidence of past Lyme disease (n = 50), patients with a range of early to late manifestations of active Lyme disease (n = 79), posttreatment Lyme disease healthy (PTLDH) individuals (n = 69), and patients with posttreatment Lyme disease syndrome (PTLDS) (n = 74). Horizontal bars indicate the mean for each group; gray section, area within 3 standard deviations of the mean for the healthy control group, above which values are considered positive. Compared with healthy controls, the active Lyme disease cohort had a significantly higher level and frequency of antibody reactivity to ECGF (P < .001). Compared with the active Lyme disease group, the level and frequency of antibody reactivity to ECGF were significantly lower in both posttreatment Lyme disease groups (PTLDS and PTLDH) (P < .001 for each). There was no significant difference in anti-ECGF antibody reactivity between the PTLDS and PTLDH cohorts (P > .99). B, Level and frequency of IgG reactivity to ECGF in healthy controls (n = 50) and in subgroups of patients with active Lyme disease, representing early to late manifestations of Lyme disease, including single erythema migrans (EM) (n = 18), multiple EM (n = 17), early neurologic (n = 16), late neurologic (n = 16), and arthritis (n = 12). Compared with the healthy cohort, there was a significantly higher level and frequency of antibody response in the multiple EM (P < .001), early neurologic (P < .001), late neurologic (P < .001), and arthritis (P < .001) groups but not the single EM group. There were no significant differences among the multiple EM, early neurologic, late neurologic, and arthritis groups. Abbreviation: OD450, optical density was measured at 450 nm.
Article Snippet: Forty-six patients were seropositive with a commercial enzyme-linked
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Journal of Alzheimer's Disease
Article Title: The Cerebrospinal Fluid Neurogranin/BACE1 Ratio is a Potential Correlate of Cognitive Decline in Alzheimer’s Disease
doi: 10.3233/JAD-160227
Figure Lengend Snippet: a) Epitope mapping of both mAbs ADxNGCI2 and ADxNGCT1 was performed with overlapping synthetic peptides, ranging from R51 to D78. b) Specificity of the mAbs ADxNGCI2 and ADxNGCT1 was evaluated by western blot analysis where both synthetic full-length neurogranin, as well as a synthetic neurogranin peptide that is truncated at P75, were included in the gel electrophoresis, next to human collagen type I, III, and IV. ADxNGCI2 positively stained both synthetic peptides, whereas ADxNGCT1 only labeled the truncated form. None of the mAbs detected the collagen proteins. c) To confirm the specificity of the prototype ELISA towards neurogranin species truncated at P75, different concentrations were analyzed of several synthetic neurogranin peptides that differ in their C-terminus, i.e., intact (Peptide intact C-term) or either truncated at P75 (Peptide trunc P75) or at S76 (Peptide trunc S76).
Article Snippet: CSF levels of total-tau, Aβ 1-42 and Aβ 1-40 were re-analyzed during this study, using the total-tau ELISA, Beta-Amyloid (1-42) ELISA and
Techniques: Western Blot, Nucleic Acid Electrophoresis, Staining, Labeling, Enzyme-linked Immunosorbent Assay
Journal: Iranian Journal of Microbiology
Article Title: Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test
doi:
Figure Lengend Snippet: The OD of sera of C. trachomatis suspected patients in rOMP2 coated ELISA plate wells.
Article Snippet: There were 7 positive samples (3.83%) and 173 negative samples (96.1%) of 180 suspected C. trachomatis sera by
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Iranian Journal of Microbiology
Article Title: Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test
doi:
Figure Lengend Snippet: The sera OD for C. trachomatis suspected patients in Euroimmun commercial ELISA kit Since the P-Value = 0.88 is greater than the significance level of a = 0.05, so there is no significant relationship between the rOMP2 and Euroimmun commercial ELISA kit.
Article Snippet: There were 7 positive samples (3.83%) and 173 negative samples (96.1%) of 180 suspected C. trachomatis sera by
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Iranian Journal of Microbiology
Article Title: Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test
doi:
Figure Lengend Snippet: Results of ELISA test with rOMP2 on 192 sera samples of C. trachomatis suspected patients.
Article Snippet: There were 7 positive samples (3.83%) and 173 negative samples (96.1%) of 180 suspected C. trachomatis sera by
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Iranian Journal of Microbiology
Article Title: Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test
doi:
Figure Lengend Snippet: Results of ELISA test with Euroimmun commercial ELISA kit on 180 sera samples of C. trachomatis suspected patients.
Article Snippet: There were 7 positive samples (3.83%) and 173 negative samples (96.1%) of 180 suspected C. trachomatis sera by
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Iranian Journal of Microbiology
Article Title: Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test
doi:
Figure Lengend Snippet: Results of ELISA with Euroimmun commercial kit and C. trachomatis rOMP2.
Article Snippet: There were 7 positive samples (3.83%) and 173 negative samples (96.1%) of 180 suspected C. trachomatis sera by
Techniques: Enzyme-linked Immunosorbent Assay
Journal: BMC Health Services Research
Article Title: Design of the Indian NCA study (Indian national collaboration on AIDS): a cluster randomized trial to evaluate the effectiveness of integrated care centers to improve HIV outcomes among men who have sex with men and persons who inject drugs in India
doi: 10.1186/s12913-016-1905-5
Figure Lengend Snippet: Data collection
Article Snippet: HSV-2 ,
Techniques: Medications, Injection, Virus, Flow Cytometry, Enzyme-linked Immunosorbent Assay
Journal: The Korean Journal of Parasitology
Article Title: Evaluation of Recombinant SAG1, SAG2, and SAG3 Antigens for Serodiagnosis of Toxoplasmosis
doi: 10.3347/kjp.2014.52.2.137
Figure Lengend Snippet: Results of IgG ELISA with recombinant SAG1, SAG2, and SAG3 in human sera. P, IgG positive; Non, non-relative (cases with different infectious diseases but not toxoplasmosis); N, negative reactions (Epstein-Barr virus, Varicella zoster virus, Herpes simplex virus, rubella, measles, Helicobacter pylori , cytomegalovirus, malaria, Echinococcus granulosus , Hymenolepis nana , Leishmania , and rheumatoid factor).
Article Snippet: Serum samples were collected from different laboratories around Shiraz and Tehran using commercial IgG and
Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Virus
Journal: The Korean Journal of Parasitology
Article Title: Evaluation of Recombinant SAG1, SAG2, and SAG3 Antigens for Serodiagnosis of Toxoplasmosis
doi: 10.3347/kjp.2014.52.2.137
Figure Lengend Snippet: Results of IgM ELISA with recombinant SAG1, SAG2, and SAG3 in human sera. P, IgM positive; N, IgM negative. Cut-off value; 0.60 (SAG1), 0.31 (SAG2), and 0.44 (SAG3), respectively.
Article Snippet: Serum samples were collected from different laboratories around Shiraz and Tehran using commercial IgG and
Techniques: Enzyme-linked Immunosorbent Assay, Recombinant